Listeria detection within 48 hours using a cultural based method
Here is an interesting product which is developed by the leading microbiology culture media brand OXOID; a listeria result in food using a modified cultural method within 48 hours. Yes that’s right it’s cultural and not one of those ELISA method. I’m not sure what the pricing is like, however if it’s cheaper than an ELISA based method, it’s a winner.
Here’s what they say:
“Oxoid Novel Enrichment Broth – Listeria (ONE Broth) and Oxoid Chromogenic Listeria Agar (OCLA) have been approved by AFNOR (Association Francaise pour la Normalisation) for the detection and differentiation of Listeria monocytogenes and other Listeria species from food samples in just 48 hours.
ONE Broth allows optimal resuscitation, recovery and growth of even low numbers of Listeria species from a single, 24-hour enrichment incubation. This saves time and materials compared to dual broth methods (such as ISO 11920-1:19971) and allows the enriched sample to be plated directly onto OCLA as soon as growth is detected.
OCLA detects ß-glucosidase activity, common to all Listeria species, and the lecithinase activity of L. monocytogenes and pathogenic L. ivanovii, allowing the detection and differentiation of L. monocytogenes and other Listeria species in only 24 hours.
Together, ONE Broth and OCLA provide presumptive identification of Listeria species in just 48 hours – up to 3 days earlier than traditional methods. The use of just one broth and a single agar plate reduces ‘hands-on’ time, frees up resources and leads to a lower cost per test. ONE Broth and OCLA were validated by AFNOR against ISO 11290-1:1997 and shown to give equivalent results2.”
